pDY118A
(Plasmid #182958)

• Purpose: pSC101 ori, chl34 resistant. Cas9 induced at 0.4 μg/ml anhydrotetracycline, recombineering proteins induced by a 15 min heat-shock at 42°C, I-Scel (induced by 0.1 mM IPTG) is to cleave the pDonor2.
• Depositing Lab: Shelley Copley
• Publication: Yang et al Proc Natl Acad Sci U S A. 2024 Jan 30;121(5):e2316834121. doi: 10.1073/pnas.2316834121. Epub 2024 Jan 22.

Backbone

• Vector backbone: Chl-resistant, SC101 ori

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol, 25 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): DH5alpha
• Growth instructions: Chl-resistant, SC101 ori, grow at 30 °C, cued at 37°C, lambda Red recombineering proteins induced at 42°C for 15 min.
• Copy number: Low Copy

Gene/Insert 1

• Gene/Insert name: Cas9
• Species: S. pyogenes
• Insert Size (bp): 4104

Gene/Insert 2

• Gene/Insert name: Gam, Beta, Exo
• Species: lambda Red

Gene/Insert 3

• Gene/Insert name: I-scel

Resource Information

• Supplemental Documents:
  • pDY118A_NGSverified.gb
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry