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Addgene

NLS-SpCas9mSA-His
(Plasmid #182951)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 182951 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    PMAX
  • Vector type
    Bacterial Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    NLS-SpCas9mSA-His

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    NLS-SpCas9mSA-His was a gift from Wen Xue (Addgene plasmid # 182951 ; http://n2t.net/addgene:182951 ; RRID:Addgene_182951)

  • For your References section:

    Genome-wide detection of CRISPR editing in vivo using GUIDE-tag. Liang SQ, Liu P, Smith JL, Mintzer E, Maitland S, Dong X, Yang Q, Lee J, Haynes CM, Zhu LJ, Watts JK, Sontheimer EJ, Wolfe SA, Xue W. Nat Commun. 2022 Jan 21;13(1):437. doi: 10.1038/s41467-022-28135-9. 10.1038/s41467-022-28135-9 PubMed 35064134
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