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pLZRS-TrkC-mCherry
(Plasmid #182949)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 182949 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    LZRSpBMN-Z
  • Backbone manufacturer
    Kinsella, T.M., and Nolan, G.P
  • Backbone size w/o insert (bp) 11652
  • Total vector size (bp) 14859
  • Modifications to backbone
    The reported LZRSpBMN-Z plasmid was digested to substitute the LacZ gene with the insert.
  • Vector type
    Mammalian Expression, Retroviral, Synthetic Biology
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    TRk-C-mCherry
  • Alt name
    NTRK3
  • Species
    R. norvegicus (rat), Synthetic
  • Insert Size (bp)
    3207
  • GenBank ID
    L14445
  • Entrez Gene
    Ntrk3 (a.k.a. trkC)
  • Promoter MMLV
  • Tag / Fusion Protein
    • mCherry (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (not destroyed)
  • 3′ cloning site NotI and XhoI (not destroyed)
  • 5′ sequencing primer pBMN 5'
  • 3′ sequencing primer L4440
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLZRS-TrkC-mCherry was a gift from Pantelis Tsoulfas (Addgene plasmid # 182949 ; http://n2t.net/addgene:182949 ; RRID:Addgene_182949)
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