pCAG-IRES2-p75NTR-GFP
(Plasmid #182947)

• Purpose: To express the rat p75NTR neurotrophin receptor. The gene contains an HA tag at the N-Terminal
• Depositing Lab: Pantelis Tsoulfas
• Publication: Neurotrophin related plasmids (unpublished)

Backbone

• Vector backbone: pCAG-IRES2
• Backbone size w/o insert (bp): 7245
• Total vector size (bp): 9255
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: p75NTR-mGFP
• Alt name: ngfr, p75, p75NTR, tnfrsf16, LNGFR
• Species: R. norvegicus (rat), Synthetic
• Insert Size (bp): 2010
• Mutation: The leader sequence is from preprotrypin
• GenBank ID: NP_036742.2 NP_036742 XP_001081319
• Promoter: CAG
• Tags / Fusion Proteins:
  • mGFP (C terminal on insert)
  • HA tag (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: none (destroyed during cloning)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: pMT-2
• 3′ sequencing primer: IRES reverse

Resource Information

• A portion of this plasmid was derived from a plasmid made by: The p75NTR was from Moses Chao

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry