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Addgene

pLV-HuGDNF
(Plasmid #182946)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 182946 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLV-MP2
  • Backbone manufacturer
    Pantelis Tsoulfas
  • Backbone size w/o insert (bp) 6762
  • Total vector size (bp) 7395
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Human GDNF
  • Alt name
    Glial Derived Neurotrophic Factor
  • Species
    H. sapiens (human), Synthetic
  • Insert Size (bp)
    633
  • GenBank ID
    NP_000505
  • Entrez Gene
    GDNF (a.k.a. ATF, ATF1, ATF2, HFB1-GDNF, HSCR3)
  • Promoter CMV enhancer

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer WPRE-R
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLV-HuGDNF was a gift from Pantelis Tsoulfas (Addgene plasmid # 182946 ; http://n2t.net/addgene:182946 ; RRID:Addgene_182946)
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