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pIN2_mcm-3_mScarlet
(Plasmid #182933)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 182933 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pIN1_GGGSx3_mScarlet
  • Backbone manufacturer
    Maureen Barr lab (Nikonorova et al 2022)
  • Backbone size w/o insert (bp) 4358
  • Total vector size (bp) 11306
  • Modifications to backbone
    insertion of genomic region Pmcm-3::mcm-3
  • Vector type
    Worm Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    genomic mcm-3 fused to mScarlet
  • Species
    C. elegans (nematode)
  • Insert Size (bp)
    3637
  • GenBank ID
    NM_074305.7
  • Promoter mcm-3
  • Tag / Fusion Protein
    • flexible linker (GGGGS)x3 and mScarlet tag (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer TCGAATGATACTAACATAACATAGAAC
  • 3′ sequencing primer TAGGGATGTTGAAGAGTAATTGGACTTAGAAGTC
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pIN2_mcm-3_mScarlet was a gift from Maureen Barr (Addgene plasmid # 182933 ; http://n2t.net/addgene:182933 ; RRID:Addgene_182933)

  • For your References section:

    Isolation, profiling, and tracking of extracellular vesicle cargo in Caenorhabditis elegans. Nikonorova IA, Wang J, Cope AL, Tilton PE, Power KM, Walsh JD, Akella JS, Krauchunas AR, Shah P, Barr MM. Curr Biol. 2022 May 9;32(9):1924-1936.e6. doi: 10.1016/j.cub.2022.03.005. Epub 2022 Mar 24. 10.1016/j.cub.2022.03.005 PubMed 35334227
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