FLAG-3C-sGFP1-10_pcDNA3.1(-)
(Plasmid
#182931)
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Purpose(Empty Backbone) For expression of membrane protein with N-terminal FLAG tag and C-terminal split GFP1-10 fragment
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 182931 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepcDNA3.1(-)
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Backbone manufacturerInvitrogen
- Backbone size (bp) 6140
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Vector typeMammalian Expression
- Promoter CMV
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Selectable markersNeomycin (select with G418)
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Tags
/ Fusion Proteins
- HA signal peptide (N terminal on insert)
- FLAG tag (N terminal on insert)
- 3C-cleavable split GFP 1-10 (C terminal on insert)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Cloning Information
- 5′ sequencing primer CMVFwd
- 3′ sequencing primer BGHRev (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
FLAG-3C-sGFP1-10_pcDNA3.1(-) was a gift from William Weis (Addgene plasmid # 182931 ; http://n2t.net/addgene:182931 ; RRID:Addgene_182931) -
For your References section:
Reconstitution of purified membrane protein dimers in lipid nanodiscs with defined stoichiometry and orientation using a split GFP tether. Bruguera ES, Mahoney JP, Weis WI. J Biol Chem. 2022 Jan 21:101628. doi: 10.1016/j.jbc.2022.101628. 10.1016/j.jbc.2022.101628 PubMed 35074428
Map uploaded by the depositor.
