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Addgene

pDEST-cDNA5-FRT/TO-3*Flag-APEX2 N-term
(Plasmid #182925)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 182925 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCDNA5/FRT/TO
  • Backbone manufacturer
    Thermofisher
  • Backbone size (bp) 7599
  • Modifications to backbone
    Backbone of APEX2 expressing vector, APEX2 was amplified from the mito-V5-APEX2 plasmid (Addgene # 72480) (Fazal et al., 2019), and in- serted into pcDNA5/FRT/TO-FLAG using the Gibson Assembly Cloning kit (NEB).
  • Vector type
    Mammalian Expression
  • Promoter CMV
  • Selectable markers
    Hygromycin ; selection after stably integrated into Flp-in cells
  • Tag / Fusion Protein
    • APEX2 and FLAG (N terminal on insert)

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol and Ampicillin, 25 & 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    ccdB Survival
  • Copy number
    Unknown

Cloning Information

  • Cloning method Gateway Cloning

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    APEX2 was amplified from the mito-V5-APEX2 plasmid (Addgene # 72480) (Fazal et al., 2019) provided by Alice Ting's lab.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDEST-cDNA5-FRT/TO-3*Flag-APEX2 N-term was a gift from Benjamin Blencowe (Addgene plasmid # 182925 ; http://n2t.net/addgene:182925 ; RRID:Addgene_182925)

  • For your References section:

    Systematic mapping of nuclear domain-associated transcripts reveals speckles and lamina as hubs of functionally distinct retained introns. Barutcu AR, Wu M, Braunschweig U, Dyakov BJA, Luo Z, Turner KM, Durbic T, Lin ZY, Weatheritt RJ, Maass PG, Gingras AC, Blencowe BJ. Mol Cell. 2022 Mar 3;82(5):1035-1052.e9. doi: 10.1016/j.molcel.2021.12.010. Epub 2022 Feb 18. 10.1016/j.molcel.2021.12.010 PubMed 35182477
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