AzoRS-4
(Plasmid
#182884)
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PurposeA pEvol-based plasmid containing one inducible (by arabinose) copy of the AzoRS-4 aaRS. The 2nd constitutive aaRS copy typically found in pEvol plasmids was deleted.
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 182884 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepEvol
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameAzoRS-4
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SpeciesSynthetic
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Insert Size (bp)921
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
AzoRS-4 was a gift from Miriam Amiram (Addgene plasmid # 182884 ; http://n2t.net/addgene:182884 ; RRID:Addgene_182884) -
For your References section:
Genetically Encoding Light-Responsive Protein-Polymers Using Translation Machinery for the Multi-Site Incorporation of Photo-Switchable Unnatural Amino Acids. Israeli B, Strugach DS, Gelkop S, Weber S, Gozlan DS, Amiram M. Adv. Funct. Mater. 2021, 31, 2011276 10.1002/adfm.202011276
