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Addgene

SNAPtag-PTS1
(Plasmid #182883)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 182883 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLJM2
  • Backbone manufacturer
    Jason Moffat
  • Backbone size w/o insert (bp) 7324
  • Total vector size (bp) 7918
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    SNAPtag-PTS1
  • Species
    H. sapiens (human), M. musculus (mouse)
  • Insert Size (bp)
    594
  • Mutation
    no
  • Promoter CMV
  • Tag / Fusion Protein
    • SNAPtag (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Nhe1 (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer CMV-R
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    SNAPtag-PTS1 was a gift from Pekka Katajisto (Addgene plasmid # 182883 ; http://n2t.net/addgene:182883 ; RRID:Addgene_182883)

  • For your References section:

    Glucose-6-phosphate-dehydrogenase on old peroxisomes maintains self-renewal of epithelial stem cells after asymmetric cell division. Bui H, Andersson S, Sola-Carvajal A, De Marchi T, Vahakangas E, Holopainen M, House AH, Rovenko BM, Englund JI, Kasper M, Kuuluvainen E, Kakela R, Hietakangas V, Nimeus E, Katajisto P. Nat Commun. 2025 Apr 26;16(1):3932. doi: 10.1038/s41467-025-58752-z. 10.1038/s41467-025-58752-z PubMed 40287409
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