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Addgene

pLV-PB2_Lock1-I436C+S993C
(Plasmid #182879)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 182879 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLENTI PGK Neo DEST
  • Backbone manufacturer
    Eric Campeau & Paul Kaufman (Addgene plasmid # 19067)
  • Backbone size w/o insert (bp) 8150
  • Total vector size (bp) 13667
  • Vector type
    Lentiviral
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    hPLXNB2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    5517
  • Mutation
    changed Isoleucine 436 to Cysteine and Serine 993 to Cysteine
  • Entrez Gene
    PLXNB2 (a.k.a. MM1, Nbla00445, PLEXB2, dJ402G11.3)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLV-PB2_Lock1-I436C+S993C was a gift from Roland Friedel (Addgene plasmid # 182879 ; http://n2t.net/addgene:182879 ; RRID:Addgene_182879)

  • For your References section:

    Invasion of glioma cells through confined space requires membrane tension regulation and mechano-electrical coupling via Plexin-B2. Junqueira Alves C, Hannah T, Sadia S, Kolsteeg C, Dixon A, Wiener RJ, Nguyen H, Tipping MJ, Silva Ladeira J, Fernandes da Costa Franklin P, de Paula Dutra de Nigro N, Alves Dias R, Zabala Capriles PV, Rodrigues Furtado de Mendonca JP, Slesinger PA, Costa KD, Zou H, Friedel RH. Nat Commun. 2025 Jan 2;16(1):272. doi: 10.1038/s41467-024-55056-6. 10.1038/s41467-024-55056-6 PubMed 39747004
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