BiDi-[mp-mEGFP]mp-mCherry2
(Plasmid
#182870)
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PurposeBi-directional expression vector of monomers with plasma membrane localization signal
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 182870 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonep210
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namemyr-palm-mEGFP, myr-palm-mCherry2
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SpeciesOther
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
- 3′ cloning site Unknown (unknown if destroyed)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
BiDi-[mp-mEGFP]mp-mCherry2 was a gift from Salvatore Chiantia (Addgene plasmid # 182870 ; http://n2t.net/addgene:182870 ; RRID:Addgene_182870) -
For your References section:
Influenza A M2 recruits M1 to the plasma membrane: A fluorescence fluctuation microscopy study. Petrich A, Dunsing V, Bobone S, Chiantia S. Biophys J. 2021 Dec 21;120(24):5478-5490. doi: 10.1016/j.bpj.2021.11.023. Epub 2021 Nov 19. 10.1016/j.bpj.2021.11.023 PubMed 34808098