pGEX-2T-TRR-C421-Y2404F
(Plasmid #182846)

• Purpose: mutation Y2404F (TAC/TTC) in GST-TRR-C421 (TRR 2011-2431 aa). Expression in E. coli. by IPTG induction
• Depositing Lab: Peter Harte
• Publication: Tie et al Development. 2014 Mar;141(5):1129-39. doi: 10.1242/dev.102392.

Backbone

• Vector backbone: pGEX-2T
• Backbone size w/o insert (bp): 4900
• Total vector size (bp): 6163
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: TRR 2011-2431
• Alt name: trithorax-related
• Species: D. melanogaster (fly)
• Insert Size (bp): 1263
• Mutation: mutation Y2404F (TAC/TTC) in GST-TRR-C421 (TRR 2011-2431 aa)
• Entrez Gene: trr (a.k.a. Dmel_CG3848, CG3848, Dmel\CG3848, EG:63B12.3, TRR, Trr, anon-2Bb, dmll3/4)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NdeI (not destroyed)
• 3′ cloning site: NsiI (not destroyed)
• 5′ sequencing primer: ATG GGTGCAGGAG CAGGCGCCAC

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The cDNA clone of TRR (SD13650) were obtained from the Drosophila Genomics Resource Center (DGRC).

Additional mutation was found at Q2050L. This is outside of the TRR SET domain (2293-2431 aa) and has no effect on its enzyme activity.