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pMT-FLAG-CBPΔ(N+HAT)
(Plasmid #182841)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 182841 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMT
  • Backbone size w/o insert (bp) 3850
  • Total vector size (bp) 7360
  • Modifications to backbone
    FLAG tag at N-terminus before NheI site
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CBP
  • Alt name
    nej
  • Species
    D. melanogaster (fly)
  • Insert Size (bp)
    3510
  • Mutation
    dletion of residues 1566-2142 from CBP1476-3222
  • Entrez Gene
    w (a.k.a. Dmel_CG2759, BACN33B1.1, CG2759, DMWHITE, DmWhite, Dmel\CG2759, EG:BACN33B1.1, W, White, c23, e(g), m(g), mini-white, mw, w(AT)[[13]])
  • Promoter MT
  • Tag / Fusion Protein
    • FLAG tag (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site NsiI (not destroyed)
  • 5′ sequencing primer gctagcaatccactg ctcct
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    The CBP cDNA clone was from Sarah Smolik

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMT-FLAG-CBPΔ(N+HAT) was a gift from Peter Harte (Addgene plasmid # 182841 ; http://n2t.net/addgene:182841 ; RRID:Addgene_182841)

  • For your References section:

    CBP-mediated acetylation of histone H3 lysine 27 antagonizes Drosophila Polycomb silencing. Tie F, Banerjee R, Stratton CA, Prasad-Sinha J, Stepanik V, Zlobin A, Diaz MO, Scacheri PC, Harte PJ. Development. 2009 Sep;136(18):3131-41. doi: 10.1242/dev.037127. 10.1242/dev.037127 PubMed 19700617
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