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Addgene

pWS3799-gRNA-Csy4-template
(Plasmid #182827)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 182827 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    ColE1
  • Vector type
    CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SpCas9 gRNA scaffold + Csy4 sequence
  • Insert Size (bp)
    100

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pWS3799-gRNA-Csy4-template was a gift from Rodrigo Ledesma-Amaro (Addgene plasmid # 182827 ; http://n2t.net/addgene:182827 ; RRID:Addgene_182827)

  • For your References section:

    Inducible expression of large gRNA arrays for multiplexed CRISPRai applications. Shaw WM, Studena L, Roy K, Hapeta P, McCarty NS, Graham AE, Ellis T, Ledesma-Amaro R. Nat Commun. 2022 Aug 25;13(1):4984. doi: 10.1038/s41467-022-32603-7. 10.1038/s41467-022-32603-7 PubMed 36008396
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