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Addgene

C321.∆A T7RNAP ompT- rne- lon-
(Bacterial strain #182778)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Bacterial Strain 182778 Bacteria in agar stab 1 $85
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Backbone

  • Vector backbone
    none

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    This is a strain.
  • Growth instructions
    Growth temperature: 32°C
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    T7, ompT, rne, lon

Cloning Information

  • Cloning method Unknown
  • 5′ sequencing primer GAACTTAATGCAACACACCA, CGTGTGCGTGTCCATAGA; CTGACAACCCCTATTGCGATCAGCTC; CAGATGGAAACCCCGCACTACCACG; CTGCGTTCCATCGTAGAAGC
  • 3′ sequencing primer CCGCGCTTAGCTTTCACT, GGTGAAGCACGCTTCC; GAGTTCAAAATCTTCATAACGATAAC; GTGCGACTACCGCTTCTTCGGCTAC; GCTGGTGCGTACTGCAATC
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    C321.∆A T7RNAP ompT- rne- lon- was a gift from Michael Jewett (Addgene plasmid # 182778)

  • For your References section:

    Improving genomically recoded Escherichia coli to produce proteins containing non-canonical amino acids. Perez JG, Carlson ED, Weisser O, Kofman C, Seki K, Des Soye BJ, Karim AS, Jewett MC. Biotechnol J. 2021 Dec 11:e2100330. doi: 10.1002/biot.202100330. 10.1002/biot.202100330 PubMed 34894206
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