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Addgene

C321.∆A ompT- rne- endA- lon-
(Bacterial strain #182775)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Bacterial Strain 182775 Bacteria in agar stab 1 $85
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Backbone

  • Vector backbone
    none

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    This is a strain.
  • Growth instructions
    Use Carbenicillin. Growth temperature: 32°C
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    ompT, rne, lon, endA

Cloning Information

  • Cloning method Unknown
  • 5′ sequencing primer CTGACAACCCCTATTGCGATCAGCTC; CAGATGGAAACCCCGCACTACCACG; CTGCGTTCCATCGTAGAAGC; ATGTACCGTTATTTGTCTATTGCTGC
  • 3′ sequencing primer GAGTTCAAAATCTTCATAACGATAAC; GTGCGACTACCGCTTCTTCGGCTAC; GCTGGTGCGTACTGCAATC; GCTGGCGCTGGTAATTTCGGCGTCA
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    C321.∆A ompT- rne- endA- lon- was a gift from Michael Jewett (Addgene plasmid # 182775)

  • For your References section:

    Improving genomically recoded Escherichia coli to produce proteins containing non-canonical amino acids. Perez JG, Carlson ED, Weisser O, Kofman C, Seki K, Des Soye BJ, Karim AS, Jewett MC. Biotechnol J. 2021 Dec 11:e2100330. doi: 10.1002/biot.202100330. 10.1002/biot.202100330 PubMed 34894206
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