C321.∆A ompT- rne- endA-
(Bacterial strain
#182774)
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PurposeRecoded E. coli MG1655 strain with UAG termination function removed (RF1 is deleted); ompT, rne, endA inactivated
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Depositing Lab
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Sequence Information
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Full plasmid sequence is not available for this item.
No maps are available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Bacterial Strain | 182774 | Bacteria in agar stab | 1 | $85 | |
Backbone
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Vector backbonenone
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)This is a strain.
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Growth instructionsUse carbenicillin. Growth temperature: 32°C
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameompT, rne, endA
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer CTGACAACCCCTATTGCGATCAGCTC; CAGATGGAAACCCCGCACTACCACG; ATGTACCGTTATTTGTCTATTGCTGC
- 3′ sequencing primer GAGTTCAAAATCTTCATAACGATAAC; GTGCGACTACCGCTTCTTCGGCTAC; GCTGGCGCTGGTAATTTCGGCGTCA (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
C321.∆A ompT- rne- endA- was a gift from Michael Jewett (Addgene plasmid # 182774) -
For your References section:
Improving genomically recoded Escherichia coli to produce proteins containing non-canonical amino acids. Perez JG, Carlson ED, Weisser O, Kofman C, Seki K, Des Soye BJ, Karim AS, Jewett MC. Biotechnol J. 2021 Dec 11:e2100330. doi: 10.1002/biot.202100330. 10.1002/biot.202100330 PubMed 34894206