pUCsRNAP
(Plasmid
#182746)
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PurposeSwapping in small RNA promoter, 23-bp spacer sequence, & 19-bp repeats into pJC005 plasmids
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 182746 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepUCsRNAP
- Backbone size w/o insert (bp) 2696
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Vector typeCRISPR
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namesmall RNA promoter, a 23-bp spacer sequence, & 19-bp repeats
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gRNA/shRNA sequenceATAATCTTTAATTTGAAAAGATTTAAGGCTTATTTAAATAAAAAATATGAGGGAAGAATTGATATAAATTTAATTTTGTTATTGTATTATGGTATGTATGGAATAAATTTAACATAAAGACAGTAATAATGTTCTTGAATTTAGACTTTTTATGTGTTATCATTAACAAGTATCAAAAATGACATTTAATAAATTAATAATAATTTTAAAAATATATTTTTGATAAAAGCAATGATTAACATGGTTTGACGTCTGAGAAGAGACGATTTTCTCAATAGGAGAAATTAAGGTGCAAacccttatcattccaccaTAATTTCTACTCTTGTAGATtcctcaaagagcatatggatatgAATTTCTACTCTTGTAGAT
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pUCsRNAP was a gift from James Collins (Addgene plasmid # 182746 ; http://n2t.net/addgene:182746 ; RRID:Addgene_182746) -
For your References section:
Rapid, Efficient, and Cost-Effective Gene Editing of Enterococcus faecium with CRISPR-Cas12a. Chua MJ, Collins J. Microbiol Spectr. 2022 Feb 2:e0242721. doi: 10.1128/spectrum.02427-21. 10.1128/spectrum.02427-21 PubMed 35107356
Map uploaded by the depositor.
