LeGO-iBSD-5LO-WT
(Plasmid #182741)

• Purpose: Lentiviral expression vector for codon-optimized 5-Lipoxygenase (WT) in mamallian cells with blasticidin S resistance (bsd).
• Depositing Lab: Dieter Steinhilber
• Publication: Kreiss et al Biochem Pharmacol. 2022 Jul 22:115187. doi: 10.1016/j.bcp.2022.115187.

Backbone

• Vector backbone: LeGI-iG2
• Backbone manufacturer: Boris Fehse
• Modifications to backbone: GFP exchanged to blasticidin S resistance (bsd)
• Vector type: Mammalian Expression, Lentiviral, Cre/Lox
• Selectable markers: Blasticidin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: 5-Lipoxygenase
• Alt name: 5-LO
• Alt name: ALOX5
• Species: H. sapiens (human)
• Insert Size (bp): 2025
• Mutation: codon optimized for expression in homo sapiens
• Entrez Gene: ALOX5 (a.k.a. 5-LO, 5-LOX, 5LPG, LOG5)
• Promoter: SFFV

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: GCCGCCACCATGCCCAGC

Resource Information

• Supplemental Documents:
  • LeGO-iBSD-5LO-WT.gb
• A portion of this plasmid was derived from a plasmid made by: LeGO-iG2 was a gift from Boris Fehse (Addgene plasmid # 27341 ; http://n2t.net/addgene:27341 ; RRID:Addgene_27341)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

HIV-1 derived third generation lentiviral vector for the expression of a cDNA. An internal ribosome entry site (IRES) is used to express the resistance gene. Use second generation (like psPAX2) or third generation (like pMDLg/pRRE + pRSV-Rev) systems for packaging in addition to a VSV-G expressing plasmid (or another envelope protein). Please visit the LeGO-Vector home page for more information: http://www.LentiGO-Vectors.de