pOSIP-KH-RBS2-dCas9
(Plasmid
#182740)
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PurposeIntegrative plasmid at the HK022 site in E. coli carrying dCas9 under the control of a Ptet promoter
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 182740 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepOSIP-HK022
- Total vector size (bp) 5203
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Vector typeCRISPR ; Integrative vector
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature30°C
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Growth Strain(s)Pir1
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Copy numberLow Copy
Gene/Insert
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Gene/Insert namedCas9
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Speciesstreptococcus pyogenes
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Insert Size (bp)4107
- Promoter Ptet
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer CTGCCAGGAATTGGGGATC (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pOSIP-KH-RBS2-dCas9 was a gift from David Bikard (Addgene plasmid # 182740 ; http://n2t.net/addgene:182740 ; RRID:Addgene_182740) -
For your References section:
Genome-wide CRISPR-dCas9 screens in E. coli identify essential genes and phage host factors. Rousset F, Cui L, Siouve E, Becavin C, Depardieu F, Bikard D. PLoS Genet. 2018 Nov 7;14(11):e1007749. doi: 10.1371/journal.pgen.1007749. eCollection 2018 Nov. 10.1371/journal.pgen.1007749 PubMed 30403660
