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Addgene

p35S-hmKO2
(Plasmid #182733)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 182733 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBRN169-MXMT
  • Vector type
    Plant Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    hmKO2
  • Alt name
    humanized monomeric Kusabira-Orange２
  • Promoter CaMV 35S promoter

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ sequencing primer GACTCTAGAGGATCCATGGTGAGCGTGATC
  • 3′ sequencing primer AGCCGGGCGGCCGCTTTAGGAGTGGGCCAC
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p35S-hmKO2 was a gift from Yutaka Kodama (Addgene plasmid # 182733 ; http://n2t.net/addgene:182733 ; RRID:Addgene_182733)

  • For your References section:

    A novel orange-colored bimolecular fluorescence complementation (BiFC) assay using monomeric Kusabira-Orange protein. Fujii Y, Yoshimura A, Kodama Y. Biotechniques. 2018 Apr;64(4):153-161. doi: 10.2144/btn-2017-0121. 10.2144/btn-2017-0121 PubMed 29661017
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