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pGWT35S-KOC169-MXMT
(Plasmid #182728)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 182728 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGWT35S
  • Modifications to backbone
    the MXMT gene was subcloned into the NcoI/SpeI site of the p35S-sfGFP vectors encoding the BiFC fragments.
  • Vector type
    Plant Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    KOC169
  • Alt name
    C-terminal fragment of mKO2, aa 169-218
  • Species
    Synthetic
  • Promoter CaMV 35S promoter

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ sequencing primer GGGGACAAGTTTGTACAAAAAAGCAGGCTTCATGGGCGGCAATCACAAA
  • 3′ sequencing primer GGGGACCACTTTGTACAAGAAAGCTGGGTCTTAGGAATGAGCTACTGCA
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGWT35S-KOC169-MXMT was a gift from Yutaka Kodama (Addgene plasmid # 182728 ; http://n2t.net/addgene:182728 ; RRID:Addgene_182728)

  • For your References section:

    A novel orange-colored bimolecular fluorescence complementation (BiFC) assay using monomeric Kusabira-Orange protein. Fujii Y, Yoshimura A, Kodama Y. Biotechniques. 2018 Apr;64(4):153-161. doi: 10.2144/btn-2017-0121. 10.2144/btn-2017-0121 PubMed 29661017
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