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Addgene

pcdna 3.1 DSG-3 Tailless Control
(Plasmid #182718)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 182718 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcdna 3.1
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    desmoglein-3
  • Alt name
    DSG3
  • Species
    M. musculus (mouse)
  • Mutation
    TSmod inserted at G726, downstream C-terminal tail of DSG-3 removed
  • GenBank ID
    NM_030596.4
  • Entrez Gene
    Dsg3 (a.k.a. bal)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    This construct was created from DSG3 Flag (received from Andrew Kowalczyk)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2024.05.03.592394 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcdna 3.1 DSG-3 Tailless Control was a gift from Daniel Conway (Addgene plasmid # 182718 ; http://n2t.net/addgene:182718 ; RRID:Addgene_182718)

  • For your References section:

    Desmosomal Cadherin Tension Loss in Pemphigus Vulgaris Mediated by the Inhibition of Active RhoA at Cell-Cell Adhesions. Jin X, Rosenbohm J, Moghaddam AO, Kim E, Seiffert-Sinha K, Leiker M, Zhai H, Baddam SR, Minnick G, Huo Y, Safa BT, Wahl JK 3rd, Meng F, Huang C, Lim JY, Conway DE, Sinha AA, Yang R. bioRxiv [Preprint]. 2024 May 6:2024.05.03.592394. doi: 10.1101/2024.05.03.592394. 10.1101/2024.05.03.592394 PubMed 38766211
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