pcdna 3.1 DSG-3 Tailless Control
(Plasmid
#182718)
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Purposeexpresses a truncated force-insensitive DSG-3 TSmod
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 182718 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcdna 3.1
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namedesmoglein-3
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Alt nameDSG3
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SpeciesM. musculus (mouse)
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MutationTSmod inserted at G726, downstream C-terminal tail of DSG-3 removed
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GenBank IDNM_030596.4
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Entrez GeneDsg3 (a.k.a. bal)
Resource Information
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A portion of this plasmid was derived from a plasmid made byThis construct was created from DSG3 Flag (received from Andrew Kowalczyk)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2024.05.03.592394 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcdna 3.1 DSG-3 Tailless Control was a gift from Daniel Conway (Addgene plasmid # 182718 ; http://n2t.net/addgene:182718 ; RRID:Addgene_182718) -
For your References section:
Desmosomal Cadherin Tension Loss in Pemphigus Vulgaris Mediated by the Inhibition of Active RhoA at Cell-Cell Adhesions. Jin X, Rosenbohm J, Moghaddam AO, Kim E, Seiffert-Sinha K, Leiker M, Zhai H, Baddam SR, Minnick G, Huo Y, Safa BT, Wahl JK 3rd, Meng F, Huang C, Lim JY, Conway DE, Sinha AA, Yang R. bioRxiv [Preprint]. 2024 May 6:2024.05.03.592394. doi: 10.1101/2024.05.03.592394. 10.1101/2024.05.03.592394 PubMed 38766211