pRS156
(Plasmid
#182707)
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Purpose(Empty Backbone) CRISPRa plasmid for Candida albicans (dCas9-VPR and sgRNA cloning site)
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 182707 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepRS143
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Vector typeYeast Expression, CRISPR
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Selectable markersNAT
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRS156 was a gift from Rebecca Shapiro (Addgene plasmid # 182707 ; http://n2t.net/addgene:182707 ; RRID:Addgene_182707) -
For your References section:
Development and applications of a CRISPR activation system for facile genetic overexpression in Candida albicans. Gervais NC, La Bella AA, Wensing LF, Sharma J, Acquaviva V, Best M, Cadena Lopez RO, Fogal M, Uthayakumar D, Chavez A, Santiago-Tirado F, Flores-Mireles AL, Shapiro RS. G3 (Bethesda). 2022 Dec 1:jkac301. doi: 10.1093/g3journal/jkac301. 10.1093/g3journal/jkac301 PubMed 36450451
Map uploaded by the depositor.
