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pORANGE_Tubb3 GFP(Y39TAG) KI
(Plasmid #182678)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 182678 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pORANGE
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Tubb3-targeting gRNA and GFP(Y39TAG) donor sequence
  • Alt name
    Tubb3
  • gRNA/shRNA sequence
    GCTGCGAGCAACTTCACTT
  • Species
    M. musculus (mouse), R. norvegicus (rat)
  • GenBank ID
    NM_023279.3 NM_139254.2
  • Entrez Gene
    Tubb3 (a.k.a. 3200002H15Rik, M(beta)3, M(beta)6)
  • Entrez Gene
    Tubb3
  • Promoter U6 and chicken beta-actin promoter

Cloning Information

  • Cloning method Unknown
  • 5′ sequencing primer aaggctgttagagagataattggaa
  • 3′ sequencing primer cgggccatttaccgtaagtt
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    This plasmid is based on the pORANGE Tubb3-GFP KI plasmid: https://www.addgene.org/131497/. We replaced WT GFP with the GFP(Y39TAG), all other sequences were unchanged.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pORANGE_Tubb3 GFP(Y39TAG) KI was a gift from Ivana Nikić-Spiegel (Addgene plasmid # 182678 ; http://n2t.net/addgene:182678 ; RRID:Addgene_182678)

  • For your References section:

    Minimal genetically encoded tags for fluorescent protein labeling in living neurons. Arsic A, Hagemann C, Stajkovic N, Schubert T, Nikic-Spiegel I. Nat Commun. 2022 Jan 14;13(1):314. doi: 10.1038/s41467-022-27956-y. 10.1038/s41467-022-27956-y PubMed 35031604
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