pLenti GW V5 Eco/Dam mse EMD
(Plasmid #182675)

• Purpose: Lentiviral eukaryotic expression vector with E.Coli Dam methylation fused to mouse Emerin. Used in DamID experiments to methylate DNA that interacts in the proximity of Emerin.
• Depositing Lab: Bas van Steensel
• Publication: Amendola et al EMBO Rep. 2015 May;16(5):610-7. doi: 10.15252/embr.201439789. Epub 2015 Mar 17.

Backbone

• Vector backbone: pLenti Gateway
• Backbone size w/o insert (bp): 8591
• Total vector size (bp): 9383
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: bleomycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Emerin
• Species: M. musculus (mouse)
• Insert Size (bp): 780
• Entrez Gene: Emd (a.k.a. AW550900, Sta)
• Promoter: HSP
• Tags / Fusion Proteins:
  • E. Coli Dam (N terminal on insert)
  • V5 (N terminal on insert)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: nvt
• 3′ sequencing primer: nvt

Resource Information

• Supplemental Documents:
  • pL-GW-EcoDam-V5-mse EMD.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry