pLenti GW V5 Eco/Dam mse LaminA
(Plasmid #182674)

• Purpose: Lentiviral eukaryotic expression vector with E.Coli Dam methylation fused to mouse Lamin A. Used in DamID experiments to methylate DNA that interacts in the proximity of Lamin A.
• Depositing Lab: Bas van Steensel
• Publication: Meuleman et al Genome Res. 2013 Feb;23(2):270-80. doi: 10.1101/gr.141028.112. Epub 2012 Nov 2.

Backbone

• Vector backbone: pLenti Gateway
• Backbone size w/o insert (bp): 8584
• Total vector size (bp): 10601
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: bleomycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Lamin A
• Species: M. musculus (mouse)
• Insert Size (bp): 1997
• Entrez Gene: Lmna (a.k.a. Dhe)
• Promoter: HSP
• Tags / Fusion Proteins:
  • E. Coli Dam (N terminal on insert)
  • V5 (N terminal on insert)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: nvt
• 3′ sequencing primer: nvt

Resource Information

• Supplemental Documents:
  • pL-GW-EcoDam-V5-mseLMNA.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry