pLenti GW V5 Eco/Dam hum LaminA
(Plasmid #182673)

• Purpose: Lentiviral eukaryotic expression vector with E.Coli Dam methylation fused to human Lamin A. Used in DamID experiments to methylate DNA that interacts in the proximity of Lamin A.
• Depositing Lab: Bas van Steensel
• Publication: Meuleman et al Genome Res. 2013 Feb;23(2):270-80. doi: 10.1101/gr.141028.112. Epub 2012 Nov 2.

Backbone

• Vector backbone: pLenti Gateway
• Backbone size w/o insert (bp): 8589
• Total vector size (bp): 10370
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: bleomycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Lamin A
• Species: H. sapiens (human)
• Insert Size (bp): 1750
• Entrez Gene: LMNA (a.k.a. CDCD1, CDDC, CMD1A, CMT2B1, EMD2, FPL, FPLD, FPLD2, HGPS, IDC, LDP1, LFP, LGMD1B, LMN1, LMNC, LMNL1, MADA, PRO1)
• Promoter: HSP
• Tags / Fusion Proteins:
  • E. Coli Dam (N terminal on insert)
  • V5 (N terminal on insert)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: nvt
• 3′ sequencing primer: nvt

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Addgene QC NGS analysis returned BLAST results with significant sequence identity to Lamin A/C.