pLenti GW V5 Eco/Dam hum LaminB1
(Plasmid
#182671)
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PurposeEukaryotic expression vector with E.Coli Dam methylation fused to human Lamin B1. Used in DamID experiments to methylate DNA that interacts in the proximity of Lamin B1.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 182671 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepLenti Gateway
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Backbone manufacturer10364
- Backbone size w/o insert (bp) 8591
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Modifications to backboneV5 - Dam
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Vector typeMammalian Expression, Lentiviral
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Selectable markersZeo marker is outside the LTRs and will not be packaged into virus.
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameLamin B1
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SpeciesH. sapiens (human)
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Insert Size (bp)1761
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Entrez GeneLMNB1 (a.k.a. ADLD, LMN, LMN2, LMNB, MCPH26)
- Promoter HSP
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Tags
/ Fusion Proteins
- E. Coli Dam (N terminal on insert)
- V5 (N terminal on insert)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer nvt
- 3′ sequencing primer nvt (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLenti GW V5 Eco/Dam hum LaminB1 was a gift from Bas van Steensel (Addgene plasmid # 182671 ; http://n2t.net/addgene:182671 ; RRID:Addgene_182671) -
For your References section:
Domain organization of human chromosomes revealed by mapping of nuclear lamina interactions. Guelen L, Pagie L, Brasset E, Meuleman W, Faza MB, Talhout W, Eussen BH, de Klein A, Wessels L, de Laat W, van Steensel B. Nature. 2008 Jun 12;453(7197):948-51. doi: 10.1038/nature06947. Epub 2008 May 7. 10.1038/nature06947 PubMed 18463634
Map uploaded by the depositor.
