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pCMV_FLAG-NFL(K211TAG)
(Plasmid #182660)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 182660 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCMV
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mouse neurofilament light chain with a K211TAG mutation
  • Alt name
    NFL(K211TAG)
  • Species
    M. musculus (mouse)
  • Mutation
    K211TAG
  • Entrez Gene
    Nefl (a.k.a. CMT2E, NF-L, NF68, Nfl)
  • Promoter CMV
  • Tag / Fusion Protein
    • FLAG tag (DYKDDDDK) (N terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV_FLAG-NFL(K211TAG) was a gift from Ivana Nikić-Spiegel (Addgene plasmid # 182660 ; http://n2t.net/addgene:182660 ; RRID:Addgene_182660)

  • For your References section:

    Minimal genetically encoded tags for fluorescent protein labeling in living neurons. Arsic A, Hagemann C, Stajkovic N, Schubert T, Nikic-Spiegel I. Nat Commun. 2022 Jan 14;13(1):314. doi: 10.1038/s41467-022-27956-y. 10.1038/s41467-022-27956-y PubMed 35031604
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