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Addgene

pcDNA3.1-CD81-(TEV)-FKBP-SmBiT
(Plasmid #182651)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 182651 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    human CD81-TEVp cleavage site-FKBP-SmBiT
  • Species
    H. sapiens (human), Synthetic
  • Entrez Gene
    CD81 (a.k.a. CVID6, S5.7, TAPA1, TSPAN28)
  • Promoter CMV
  • Tag / Fusion Protein
    • SmBiT (C terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1-CD81-(TEV)-FKBP-SmBiT was a gift from Masaharu Somiya (Addgene plasmid # 182651 ; http://n2t.net/addgene:182651 ; RRID:Addgene_182651)

  • For your References section:

    Engineering of Extracellular Vesicles for Small Molecule-Regulated Cargo Loading and Cytoplasmic Delivery of Bioactive Proteins. Somiya M, Kuroda S. Mol Pharm. 2022 May 20. doi: 10.1021/acs.molpharmaceut.2c00192. 10.1021/acs.molpharmaceut.2c00192 PubMed 35594496
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