pxpr_502-wt-ltr
(Plasmid
#182647)
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Purposefor CRISPRa, lentiviral expression of gRNA scaffold using activator P65 HSF, contains intact LTR
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 182647 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepXPR_502
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Modifications to backboneAdded functional 3'-LTR sequence
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Vector typeMammalian Expression, Lentiviral, CRISPR
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameNone
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SpeciesSynthetic
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pxpr_502-wt-ltr was a gift from Jakob Reiser (Addgene plasmid # 182647 ; http://n2t.net/addgene:182647 ; RRID:Addgene_182647) -
For your References section:
CRISPR library screening to develop HEK293-derived cell lines with improved lentiviral vector titers. Iaffaldano BJ, Marino MP, Reiser J. Front Genome Ed. 2023 Jul 13;5:1218328. doi: 10.3389/fgeed.2023.1218328. eCollection 2023. 10.3389/fgeed.2023.1218328 PubMed 37520398
Map uploaded by the depositor.
