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pUC57-RPLP15UTR-hRluc-A60
(Plasmid #182639)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 182639 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUC57
  • Backbone size w/o insert (bp) 2840
  • Total vector size (bp) 3926
  • Vector type
    Mammalian Expression, Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Renilla luciferase
  • Alt name
    RLuc
  • Species
    Renilla reniformis
  • Mutation
    The transcription is driven by a T7 promoter, paired with 165 bp 5'UTR from RPLP1
  • GenBank ID
    AAG54094.1

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Floor and Doudna, ELife, 2016

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pUC57-RPLP15UTR-hRluc-A60 was a gift from Stephen Floor (Addgene plasmid # 182639 ; http://n2t.net/addgene:182639 ; RRID:Addgene_182639)

  • For your References section:

    DDX3 depletion represses translation of mRNAs with complex 5' UTRs. Calviello L, Venkataramanan S, Rogowski KJ, Wyler E, Wilkins K, Tejura M, Thai B, Krol J, Filipowicz W, Landthaler M, Floor SN. Nucleic Acids Res. 2021 May 21;49(9):5336-5350. doi: 10.1093/nar/gkab287. 10.1093/nar/gkab287 PubMed 33905506
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