pUC57-min5UTR-hRluc-A60
(Plasmid
#182638)
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PurposeTranscription template plasmid for a minimal 5'UTR followed by Renilla luciferase open reading frame and polyA sequence
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 182638 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepUC57
- Backbone size w/o insert (bp) 2840
- Total vector size (bp) 3770
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Vector typeMammalian Expression, Luciferase
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameRenilla luciferase,
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Alt nameRLuc
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SpeciesRenilla reniformis
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GenBank IDAAG54094.1
Resource Information
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A portion of this plasmid was derived from a plasmid made byFloor and Doudna, ELife, 2016
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pUC57-min5UTR-hRluc-A60 was a gift from Stephen Floor (Addgene plasmid # 182638 ; http://n2t.net/addgene:182638 ; RRID:Addgene_182638) -
For your References section:
DDX3 depletion represses translation of mRNAs with complex 5' UTRs. Calviello L, Venkataramanan S, Rogowski KJ, Wyler E, Wilkins K, Tejura M, Thai B, Krol J, Filipowicz W, Landthaler M, Floor SN. Nucleic Acids Res. 2021 May 21;49(9):5336-5350. doi: 10.1093/nar/gkab287. 10.1093/nar/gkab287 PubMed 33905506