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lentiguide-puro-wt-ltr
(Plasmid #182635)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 182635 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    lentiGuide-Puro
  • Modifications to backbone
    Added functional 3'-LTR sequence
  • Vector type
    Mammalian Expression, Lentiviral, CRISPR
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    S. pyogenes sgRNA cassette
  • Species
    Synthetic
  • Promoter hU6

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BsmBI (not destroyed)
  • 3′ cloning site BsmBI (not destroyed)
  • 5′ sequencing primer hU6-F
  • 3′ sequencing primer hGata4-rev (5'-ATTGTGGATGAATACTGCC-3')
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    lentiguide-puro-wt-ltr was a gift from Jakob Reiser (Addgene plasmid # 182635 ; http://n2t.net/addgene:182635 ; RRID:Addgene_182635)

  • For your References section:

    CRISPR library screening to develop HEK293-derived cell lines with improved lentiviral vector titers. Iaffaldano BJ, Marino MP, Reiser J. Front Genome Ed. 2023 Jul 13;5:1218328. doi: 10.3389/fgeed.2023.1218328. eCollection 2023. 10.3389/fgeed.2023.1218328 PubMed 37520398
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