pBABR IRFP-Bcd-LEXY
(Plasmid
#182595)
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PurposeDrosophila integration plasmid expressing IRFP-Bcd-LEXY fusion protein under the maternal tubulin promoter.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 182595 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepBABR
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Vector typeInsect Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameBcd
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SpeciesD. melanogaster (fly)
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Entrez Genebcd (a.k.a. Dmel_CG1034, BCD, BG:DS00276.7, Bcd, Bicoid, CG1034, Dm-Bcd, Dmel\CG1034, bic, mum, prd4)
- Promoter maternal tubulin
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Tags
/ Fusion Proteins
- LEXY (C terminal on insert)
- IRFP (N terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer a
- 3′ sequencing primer a (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBABR IRFP-Bcd-LEXY was a gift from Jared Toettcher (Addgene plasmid # 182595 ; http://n2t.net/addgene:182595 ; RRID:Addgene_182595) -
For your References section:
Optogenetic control of the Bicoid morphogen reveals fast and slow modes of gap gene regulation. Singh AP, Wu P, Ryabichko S, Raimundo J, Swan M, Wieschaus E, Gregor T, Toettcher JE. Cell Rep. 2022 Mar 22;38(12):110543. doi: 10.1016/j.celrep.2022.110543. 10.1016/j.celrep.2022.110543 PubMed 35320726
