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Addgene

pGWT35S-TIM21(1–50)-Citrine
(Plasmid #182584)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 182584 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGWT35S
  • Vector type
    Plant Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    TIM21(1–50)
  • Alt name
    translocase of inner mitochondrial membrane 21 (1–50)
  • Promoter CaMV 35S promoter
  • Tag / Fusion Protein
    • Citrine (C terminal on insert)

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ sequencing primer GGGGACAAGTTTGTACAAAAAAGCAGGCTCCATGATGATGATGAATCT
  • 3′ sequencing primer GGGGACCACTTTGTACAAGAAAGCTGGGTCTCACTTGTACAGCTCGTCC
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGWT35S-TIM21(1–50)-Citrine was a gift from Yutaka Kodama (Addgene plasmid # 182584 ; http://n2t.net/addgene:182584 ; RRID:Addgene_182584)

  • For your References section:

    Particle bombardment and subcellular protein localization analysis in the aquatic plant Egeria densa. Osaki Y, Kodama Y. PeerJ. 2017 Sep 7;5:e3779. doi: 10.7717/peerj.3779. eCollection 2017. 10.7717/peerj.3779 PubMed 28894649
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