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Addgene

LifeAct-GFP-Fis1
(Plasmid #182576)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 182576 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEGFP
  • Backbone size w/o insert (bp) 3963
  • Total vector size (bp) 4853
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    LifeAct-GFP-Fis1
  • Insert Size (bp)
    890
  • Tags / Fusion Proteins
    • LifeAct (N terminal on insert)
    • Fis1 (C terminal on insert)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    LifeAct-GFP-Fis1 was a gift from Uri Manor (Addgene plasmid # 182576 ; http://n2t.net/addgene:182576 ; RRID:Addgene_182576)

  • For your References section:

    Actin chromobody imaging reveals sub-organellar actin dynamics. Schiavon CR, Zhang T, Zhao B, Moore AS, Wales P, Andrade LR, Wu M, Sung TC, Dayn Y, Feng JW, Quintero OA, Shadel GS, Grosse R, Manor U. Nat Methods. 2020 Sep;17(9):917-921. doi: 10.1038/s41592-020-0926-5. Epub 2020 Aug 10. 10.1038/s41592-020-0926-5 PubMed 32778832
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