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Addgene

FnR8-10
(Plasmid #182569)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 182569 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET21
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Human fibronectin fragment, type-III repeats 8-10
  • Alt name
    FnR8-10
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    879
  • Mutation
    R1379A
  • Tags / Fusion Proteins
    • His (N terminal on insert)
    • TEV cleavage site after His tag (N terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    FnR8-10 was a gift from Timothy Springer (Addgene plasmid # 182569 ; http://n2t.net/addgene:182569 ; RRID:Addgene_182569)

  • For your References section:

    Dynamics of integrin alpha5beta1, fibronectin, and their complex reveal sites of interaction and conformational change. Su Y, Iacob RE, Li J, Engen JR, Springer TA. J Biol Chem. 2022 Sep;298(9):102323. doi: 10.1016/j.jbc.2022.102323. Epub 2022 Aug 2. 10.1016/j.jbc.2022.102323 PubMed 35931112
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