FnR9-10
(Plasmid
#182567)
-
PurposeHuman fibronectin fragment, type-III repeats 9-10, R1379A mutant
-
Depositing Lab
-
Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 182567 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
-
Vector backbonepET21
-
Vector typeBacterial Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberLow Copy
Gene/Insert
-
Gene/Insert nameHuman fibronectin fragment, type-III repeats 9-10
-
Alt nameFnR9-10
-
SpeciesH. sapiens (human)
-
Insert Size (bp)612
-
MutationR1379A
-
Tags
/ Fusion Proteins
- His (N terminal on insert)
- TEV cleavage site after His tag (N terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer T7 Promoter
- 3′ sequencing primer T7 Terminator (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
FnR9-10 was a gift from Timothy Springer (Addgene plasmid # 182567 ; http://n2t.net/addgene:182567 ; RRID:Addgene_182567) -
For your References section:
Dynamics of integrin alpha5beta1, fibronectin, and their complex reveal sites of interaction and conformational change. Su Y, Iacob RE, Li J, Engen JR, Springer TA. J Biol Chem. 2022 Sep;298(9):102323. doi: 10.1016/j.jbc.2022.102323. Epub 2022 Aug 2. 10.1016/j.jbc.2022.102323 PubMed 35931112
