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Addgene

T4 Lysozyme L31d-3=L31d WT*
(Plasmid #18256)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 18256 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pHS1403
  • Backbone size w/o insert (bp) 5000
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    T4 Lysozyme
  • Alt name
    L31d-3=L31d
  • Species
    Bacteriophage T4
  • Insert Size (bp)
    600
  • Mutation
    insertion of Gly Ile Gly His Leu Leu after I27 WT*
  • GenBank ID
    NP_049736
  • Entrez Gene
    e (a.k.a. T4p123)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer na
  • 3′ sequencing primer GGCGTATCACGAGGCCC
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The BamHI site is 27 bp from the ATG and the HindIII site is 115 bp from the stop.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    T4 Lysozyme L31d-3=L31d WT* was a gift from Brian Matthews (Addgene plasmid # 18256 ; http://n2t.net/addgene:18256 ; RRID:Addgene_18256)

  • For your References section:

    Sequential reorganization of beta-sheet topology by insertion of a single strand. Sagermann M, Baase WA, Matthews BW. Protein Sci. 2006 May . 15(5):1085-92. 10.1110/ps.052018006 PubMed 16597830
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