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Addgene

ER-GCaMP6f
(Plasmid #182548)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 182548 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA 3.1
  • Backbone size w/o insert (bp) 5308
  • Total vector size (bp) 5326
  • Vector type
    Mammalian Expression, Bacterial Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    ER localized P450
  • Insert Size (bp)
    81

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    ER-GCaMP6f was a gift from Chris Richards (Addgene plasmid # 182548 ; http://n2t.net/addgene:182548 ; RRID:Addgene_182548)

  • For your References section:

    ER-GCaMP6f: An Endoplasmic Reticulum-Targeted Genetic Probe to Measure Calcium Activity in Astrocytic Processes. Aryal SP, Xia M, Adindu E, Davis C, Ortinski PI, Richards CI. Anal Chem. 2022 Jan 21. doi: 10.1021/acs.analchem.1c04321. 10.1021/acs.analchem.1c04321 PubMed 35061939
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