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pCD5-H5_Vietnam_1203/04_Y161A_sfGFP_ST
(Plasmid #182547)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 182547 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCD5
  • Backbone size w/o insert (bp) 3400
  • Total vector size (bp) 6900
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin and Tetracycline, 100 & 10 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    MC1061
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    H5_Vietnam_1203_04_Y161A
  • Insert Size (bp)
    1700
  • Mutation
    Y161A mutant that confers exclusive NeuAc to NeuGc specificity
  • Promoter CMV
  • Tag / Fusion Protein
    • GCN4-TEV-sfGFP-TwinStrep (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site PacI (not destroyed)
  • 5′ sequencing primer GGC TAA CTA GAG AAC CCA CTG C
  • 3′ sequencing primer TTC AGG GGG AGG TGT GGG AG
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCD5-H5_Vietnam_1203/04_Y161A_sfGFP_ST was a gift from Robert de Vries (Addgene plasmid # 182547 ; http://n2t.net/addgene:182547 ; RRID:Addgene_182547)

  • For your References section:

    N-Glycolylneuraminic Acid as a Receptor for Influenza A Viruses. Broszeit F, Tzarum N, Zhu X, Nemanichvili N, Eggink D, Leenders T, Li Z, Liu L, Wolfert MA, Papanikolaou A, Martinez-Romero C, Gagarinov IA, Yu W, Garcia-Sastre A, Wennekes T, Okamatsu M, Verheije MH, Wilson IA, Boons GJ, de Vries RP. Cell Rep. 2019 Jun 11;27(11):3284-3294.e6. doi: 10.1016/j.celrep.2019.05.048. 10.1016/j.celrep.2019.05.048 PubMed 31189111
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