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Addgene

pEVOL-MmAcKRS1-PylTUUA
(Plasmid #182537)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 182537 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEVOL
  • Total vector size (bp) 5651
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    PylTUUA
  • Alt name
    Pyrrolysyl-tRNA-UUA
  • Species
    methanosarcina mazei
  • Insert Size (bp)
    72
  • Promoter ProK

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEVOL-MmAcKRS1-PylTUUA was a gift from Wenshe Liu (Addgene plasmid # 182537 ; http://n2t.net/addgene:182537 ; RRID:Addgene_182537)

  • For your References section:

    Catalyst-free and site-specific one-pot dual-labeling of a protein directed by two genetically incorporated noncanonical amino acids. Wu B, Wang Z, Huang Y, Liu WR. Chembiochem. 2012 Jul 9;13(10):1405-8. doi: 10.1002/cbic.201200281. Epub 2012 May 24. 10.1002/cbic.201200281 PubMed 22628069
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