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Addgene

T7-Phi6-P2
(Plasmid #182535)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 182535 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    T7-CMVtrans-FFLuc-polyA
  • Backbone size w/o insert (bp) 3703
  • Total vector size (bp) 5177
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    P2 pol
  • Species
    Pseudomonas virus phi6
  • Insert Size (bp)
    1474
  • GenBank ID
    NC_003715.1
  • Promoter T7 promoter

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer TAATACGACTCACTATAGGG
  • 3′ sequencing primer GGTAGGGCGACCAAGAACTT
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    T7-Phi6-P2 was a gift from Peter Sarin (Addgene plasmid # 182535 ; http://n2t.net/addgene:182535 ; RRID:Addgene_182535)

  • For your References section:

    An improved RT-qPCR method for direct quantification of enveloped RNA viruses. Gregorova P, Heinonen MK, Sarin LP. MethodsX. 2022 May 23;9:101737. doi: 10.1016/j.mex.2022.101737. eCollection 2022. 10.1016/j.mex.2022.101737 PubMed 35669085
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