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Addgene

pDRF1-GW AKAR3-EV
(Plasmid #182533)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 182533 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDRF1-GW
  • Total vector size (bp) 9156
  • Vector type
    Yeast Expression
  • Selectable markers
    URA3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    AKAR3-EV
  • Species
    Synthetic
  • Promoter PMA1

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (unknown if destroyed)
  • 3′ cloning site BamHI (unknown if destroyed)
  • 5′ sequencing primer AACAATCGTTAATAATTAATTAATTGG
  • 3′ sequencing primer GAGTCACTTTAAAATTTGTATACAC
    • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Dr. Miyazaki and Kazuhiro AOKI

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

AKAR3-EV is obtained from https://doi.org/10.1091/mbc.e11-01-0072.

Please visit https://doi.org/10.1101/2022.10.27.514151 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDRF1-GW AKAR3-EV was a gift from Bas Teusink (Addgene plasmid # 182533 ; http://n2t.net/addgene:182533 ; RRID:Addgene_182533)

  • For your References section:

    Using the AKAR3-EV biosensor to assess Sch9p- and PKA-signalling in budding yeast. Botman D, Kanagasabapathi S, Savakis P, Teusink B. FEMS Yeast Res. 2023 Jan 4;23:foad029. doi: 10.1093/femsyr/foad029. 10.1093/femsyr/foad029 PubMed 37173282
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