pAAV-TRE-DIO-hM4Di-mCherry
(Plasmid #182532)

• Purpose: This plasmid is for use with neuronal cell-type selective activity tagging. The hM4Di receptor expression requires both neural activity and Cre recombinase.
• Depositing Lab: William Wisden
• Publication: Yu et al Science. 2022 Jul;377(6601):63-72. doi: 10.1126/science.abn0853. Epub 2022 Jun 30.

Backbone

• Vector backbone: pUC
• Backbone size w/o insert (bp): 2900
• Total vector size (bp): 6828
• Vector type: Mammalian Expression, Mouse Targeting, AAV, Cre/Lox

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: AAV transgene - hSyn promoter-tet-flex-hM4Di-cherry
• Species: Synthetic
• Insert Size (bp): 4076
• Promoter: tetO promoter
• Tag / Fusion Protein:
  • hM4Di-mCherry

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: catcgtggaacagtacgaac
• 3′ sequencing primer: caacttcacacctgtcaatg

Resource Information

• A portion of this plasmid was derived from a plasmid made by: The hM4di-Cherry part of the insert was a gift from Bryan L. Roth, Addgene plasmid #50475

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry