NAT-TET-OFF -MET - 3xHA
(Plasmid
#182526)
-
PurposeDoxycycline-induced transcriptional regulation and 3xHA N-terminal tagging.
-
Depositing Lab
-
Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 182526 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
-
Vector backboneUnknown
- Total vector size (bp) 4919
-
Vector typeYeast Expression
-
Selectable markersNAT
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberUnknown
Gene/Insert
-
Gene/Insert name7 Copies of TetO Box
-
Tag
/ Fusion Protein
- 3HA (N terminal on backbone)
Resource Information
-
Supplemental Documents
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
NAT-TET-OFF -MET - 3xHA was a gift from Rob Mitra (Addgene plasmid # 182526 ; http://n2t.net/addgene:182526 ; RRID:Addgene_182526) -
For your References section:
A Suite of New Strain Construction Vectors for Gene Expression Knockdown in Budding Yeast. Shively CA, Dong F, Mitra RD. ACS Synth Biol. 2023 Jan 17. doi: 10.1021/acssynbio.2c00547. 10.1021/acssynbio.2c00547 PubMed 36650116
