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Addgene

SIR4 gRNA HIS
(Plasmid #182520)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 182520 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRS413
  • Total vector size (bp) 6161
  • Vector type
    Yeast Expression
  • Selectable markers
    HIS3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    SIR4 gRNA
  • gRNA/shRNA sequence
    CCATCAAAGAGGCCCCAGCT
  • Species
    S. cerevisiae (budding yeast)
  • Entrez Gene
    SIR4 (a.k.a. YDR227W, ASD1, STE9, UTH2)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    SIR4 gRNA HIS was a gift from Rob Mitra (Addgene plasmid # 182520 ; http://n2t.net/addgene:182520 ; RRID:Addgene_182520)

  • For your References section:

    A Suite of New Strain Construction Vectors for Gene Expression Knockdown in Budding Yeast. Shively CA, Dong F, Mitra RD. ACS Synth Biol. 2023 Jan 17. doi: 10.1021/acssynbio.2c00547. 10.1021/acssynbio.2c00547 PubMed 36650116
Included in kit:
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